Characterization of microsatellite loci in the lichen fungus Lobaria pulmonaria (Lobariaceae)1
نویسندگان
چکیده
UNLABELLED PREMISE OF THE STUDY Microsatellite loci were developed for the threatened haploid lichen fungus Lobaria pulmonaria to increase the resolution to identify clonal individuals, and to study its population subdivision. • METHODS AND RESULTS We developed 14 microsatellite markers from 454 DNA sequencing data of L. pulmonaria and tested for cross-amplification with L. immixta and L. macaronesica. The number of alleles per locus ranged from two to 23. Nei's unbiased gene diversity, averaged over loci, ranged from 0.434 to 0.517 in the three studied populations. • CONCLUSIONS The new markers will increase the genetic resolution in studies that aim at disentangling clones in L. pulmonaria and may be useful for closely related species within Lobaria sect. Lobaria.
منابع مشابه
Characterization of microsatellite loci in the Himalayan lichen fungus Lobaria pindarensis (Lobariaceae)1
UNLABELLED PREMISE OF THE STUDY Microsatellite loci were developed for the rare, Himalayan, endemic haploid lichen fungus, Lobaria pindarensis, to study its population subdivision and the species' response to forest disturbance and fragmentation. • METHODS AND RESULTS We developed 18 polymorphic microsatellite markers using 454 pyrosequencing data and assessed them in 109 individuals. The ...
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